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GPV distribution in the tissues of ducklings was assessed by amplifying an approximately 400-bp fragment of the GPV genome. This fragment bands could be detected in the liver, lung, jejunum, and ileum during the observation period (Table 3). The 400 bp DNA band could be always detected in cardiac muscle, bursa of Fabricius, duodenum, rectum, brain, kidney and skeletal muscle at 24, 48, 96, 144, 192 h PI (Table 3). The DNA bands in skeletal and cardiac muscle were especially bright. (Additional file 1: Figure S1). Viral DNA band could not be detected in the pancreas and large intestine (Additional file 1: Figure S1). These results suggested that GPVwas primarily remained in muscle.

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